General Media and Vessels Suitable for Woody Plant Culture

  • B. H. Mccown
  • J. C. Sellmer
Part of the Forestry Sciences book series (FOSC, volume 24-26)


The factors to be considered when microculturing a plant tissue generally can be grouped into five broad categories: 1) tissue (genotype, source, and history), 2) media (minerals, hormones and other organics, supporting agents), 3) environment (light, temperature, gases, vessels), 4) timing (subculture period, dosage), and 5) interactions between the above factors. Each investigator has a bias as to the relative importance of each of these, however no factor can be considered separately from the others. This makes the last factor, interactions, one of the most important but because of the difficulties in conducting and interpreting multifactor experiments, interactions are also the least studied and most poorly understood. Thus a chapter with a goal of addressing one factor is necessarily limited in scope.


Shoot Culture Woody Plant Medium Calcium Deficiency Vessel Type Vessel Characteristic 
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© Springer Science+Business Media Dordrecht 1987

Authors and Affiliations

  • B. H. Mccown
  • J. C. Sellmer

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