Structure of Cell Organelles and Cell Wall in Tissue Cultures of Trees

  • Liisa Kaarina Simola
Part of the Forestry Sciences book series (FOSC, volume 24-26)


Some observations of the structure and development of cell organelles and cell wall have been included in the early experimental works on tissue cultures of trees (2, 3, 17). Differentiation of tracheary and sieve elements was the main aim in several light microscopic studies. The importance of carbohydrate level as well as the role of auxin in cytodifferentiation was determined using callus cultures of Syringa vulgaris (47, 48). Sucrose which was used as sugar in these experiments was favourable for sieve element development at a rather high concentration (4–5%). The same was the case in tissue cultures of Camellia (19). Raffinose and lactose, however, were the most effective carbohydrate sources in the induction of vascularized nodules in Sequoia callus (3).


Suspension Culture Callus Culture Rough Endoplasmic Reticulum Callus Line Sieve Element 
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  1. 1.
    BAGSHAW V, K BROWN, MM YEOMAN 1969 Changes in the mitochondrial complex accompanying callus growth. Ann Bot 33: 35–44Google Scholar
  2. 2.
    BALL E 1950 Differentiation in a callus culture of Sequoia sempervirens. Growth 14: 295–323Google Scholar
  3. 3.
    BALL E 1955 Studies on the nutrition of the callus culture of Sequoia sempervirens. Anne Biol 59: 281–305Google Scholar
  4. 4.
    BARNETT JR 1977 Fine structure of parenchymatous and differentiated Pinus radiata callus. Ann Bot 42: 367–379Google Scholar
  5. 5.
    BARNETT JR 1981 Secondary xylem cell development. In JR Barnett, ed, Xylem Cell Development. Castle House Publ Great Britain, pp 47–95Google Scholar
  6. 6.
    BAUR PS, CH WALKINSHAW 1974 Fine structure of tannin accumulation in callus culture of Pious elliotti (slash pine). Can J Bot 52: 615–619CrossRefGoogle Scholar
  7. 7.
    BORCHERT R 1968 Spontane Diploidisierung in Gewebekulturen des Megagametophyten von Pinus Lambertiana. Z Pflanzenphysiol 59: 389–392Google Scholar
  8. 8.
    BORNMAN CH 1974 Cytodifferentiation in tissue culture. In HE Street, ed, Tissue Culture and Plant Science. Academic Press, London, pp 43–70Google Scholar
  9. 9.
    CARDEMIL L, M JORDAN 1982 Light and electron microscopic study in vitro cultured female gametophyte of Araucaria araucana ( Mol.) Koch. Z Pflanzenphysiol 107: 329–338Google Scholar
  10. 10.
    CHAFE SC, DJ DURZAN 1973 Tannin inclusions in cell suspension cultures of white spruce. Planta 113: 251–262CrossRefGoogle Scholar
  11. 11.
    COX GC, BE JUNIPER 1973 Autoradiographie evidence for paramural body function. Nature 243: 116–117Google Scholar
  12. 12.
    CRONSHAW J 1964 Crystal containing bodies of plant cells. Protoplasma 59: 318–325CrossRefGoogle Scholar
  13. 13.
    D’AMATO F 1978 Chromosome number variation in cultured cells and regenerated plants. In TA Thorpe, ed, Frontiers of Plant Tissue Culture 1978: Int Assoc Plant Tissue Cult, Univ Calgary, Alberta, Canada, pp 287–295Google Scholar
  14. 14.
    DAVEY MR, HE STREET 1971 Studies on the growth in culture of plant cells. IX Additional features of the fine structure of Acer pseudoplatanus L. cells cultured in suspension. J Exp Bot 22: 90–94CrossRefGoogle Scholar
  15. 15.
    DURZAN DJ, SC CHAFE, SM LOPUHANSKI 1973 Effect of environmental changes on sugar, tannins and organized growth in suspension cultures of white spruce. Planta 113: 241–249CrossRefGoogle Scholar
  16. 16.
    FOWKE LC, G SETTERFIELD 1969 Multivesicular structures and cell wall growth. Can J Bot 47: 1873–1877CrossRefGoogle Scholar
  17. 17.
    GAUTHERET RJ 1959 Culture des Tissus Végétaux: Techniques et Realisations. Massons et Cie, ParisGoogle Scholar
  18. 18.
    HARRIS N 1981 Plasmalemmasomes in cotyledon leaves of germinating Vigna radiata L. (mung beans). Plant Cell Environ 4: 169–175CrossRefGoogle Scholar
  19. 19.
    JEFFS RA, DH NORTHCOTE 1967 The influence of indol3-ylacetic acid and sugar on the pattern of induced differentiation in plant tissue culture. J Cell Sci 2: 77–88PubMedGoogle Scholar
  20. 20.
    JENSEN TE, JG VALDOVINOS 1967 Fine structure of abscission zones 1. Abscission zones of the pedicels of tobacco and tomato flower at anthesis. Planta 77: 298–318Google Scholar
  21. 21.
    NEWCOMB EH, SE FREDERICK 1971 Distribution and structure of plant microbodies (peroxisomes). In MD Hatch, CB Osmond, RO Slatyer, eds, Photosynthesis and Photorespiration, Wiley-Interscience, New York, pp 442–457Google Scholar
  22. 22.
    PARHAM RA, HM KAUSTINEN 1977 On the site of tannin synthesis in plant cell. Bot Gaz 138: 465–467CrossRefGoogle Scholar
  23. 23.
    PARTANEN CR 1963 Plant tissue culture in relation to developmental cytology. Int Rev Cytol 15: 215–243PubMedCrossRefGoogle Scholar
  24. 24.
    ROBERTS K, DH NORTHCOTE 1970 The structure of sycamore callus cells during division in partially synchronised suspension culture. J Cell Sci 6: 299–321PubMedGoogle Scholar
  25. 25.
    ROHR R 1978 Ultrastructure du tissu haploide de Ginkgo biloba L. Ultrastructure of the haploid tissue of Ginkgo biloba L. Z Pflanzenphysiol 87: 365–378Google Scholar
  26. 26.
    ROHR R 1979 Reconstruction tridimensionelle du chondriome de Ginkgo en cultures tissulaires: étude au moyen de coupes épaisses et de coupes fines sériées. Can J Bot 57: 332–340CrossRefGoogle Scholar
  27. 27.
    ROHR R 1980 Dédifferenciation en culture in vitro des cellules â reserves dans l’endosperme de Ginkgo biloba. Z Pflanzenphysiol 96: 423–434Google Scholar
  28. 28.
    ROHR R 1982 Activation et prolifération des cellules du mégagamétophyte de Taxus cultivé in vitro. Can J Bot 60: 1583–1589CrossRefGoogle Scholar
  29. 29.
    SALMIA MA 1975 Cytological studies on tissue culture of Pinus cembra. Physiol Plant 33: 58–61Google Scholar
  30. 30.
    SIMOLA LK 1972 Changes in the ultrastructure of cells of Atropa belladonna cv. lutea Döll. during growth and differentiation in suspension culture. Z Pflanzenphysiol 68: 215–227Google Scholar
  31. 31.
    SIMOLA LK 1975 Changes in the subcellular organization of endosperm and radicle cells of Picea abies during germination. Z Pflanzenphysiol 78: 41–51Google Scholar
  32. 32.
    SIMOLA LK 1982a Ultrastructure of callus cultures from trees. In Proc IUFRO Sect S2 01 5. Int Workshop In Vitro“ Cultivation For Tree Species, Fontainebleau, France, 1981Google Scholar
  33. 33.
    SIMOLA LK 1982b Ultrastructure of callus cultures from Betula pendula and Picea abies. In A Fujiwara, ed, Plant Tissue Culture 1982. Proc 5th Int Cong Plant Tissue Cult, Tokyo,Japan, pp 173–174Google Scholar
  34. 34.
    SIMOLA LK 1982c Electron microscope observations on the differentiation of protocorm cells of Dactylorhiza maculata. Nord J Bot 2: 125–130Google Scholar
  35. 35.
    SIMOLA LK 1985 Propagation of plantlets from leaf callus of Betula pendula f. purpurea. Sci Hortic 26: 77–85Google Scholar
  36. 36.
    SIMOLA LK, J HONKANEN 1983 Organogenesis and fine structure in megagametophyte callus lines of Picea abies. Physiol Plant 59: 551–561Google Scholar
  37. 37.
    SIMOLA LK, K KOSKIMIES-SOININEN 1984 Comparison of glycolipids and plastids in callus cells and leaves of Alnus and Betula. Plant Cell Physiol 25: 1329–1340Google Scholar
  38. 38.
    SINGH AP 1984 Pinus radiata needle trace studies: fine structure of immature sieve cells in the primary xylem. Cytologia 49: 359–384CrossRefGoogle Scholar
  39. 39.
    STREET HE, MR DAVEY, B SUTTON-JONES 1972 Ultra-structure of plant cells growing in suspension culture. Symp Biol Hung 14: 145–159Google Scholar
  40. 40.
    SUSSEX IM, MG CLUTTER 1967 Differentiation in tissues, free cells, and reaggregated plant cells. In Vitro 3: 3–12Google Scholar
  41. 41.
    SUTTON-JONES B, HE STREET 1968 Studies on the growth in culture of plant cells III. Changes in fine structure during the growth of Acer ppeudoplatanus L. cells in suspension culture. J Exp Bot 19: 114–118.CrossRefGoogle Scholar
  42. 42.
    TORREY J 1967 Morphogenesis in relation to chromosomal constitution in long term plant tissue culture. Physiol Plant 20: 265–275CrossRefGoogle Scholar
  43. 43.
    TULECKE W 1957 The pollen of Ginkgo biloba: In vitro culture and tissue formation. Am J Bot 44: 602–608CrossRefGoogle Scholar
  44. 44.
    TULECKE W 1967 Studies on tissue culture derived from Ginkgo biloba. Phytomorphology 17: 381–386Google Scholar
  45. 45.
    VERBELEN J-P 1977 A tubular type of plasmalemmasome in xylem of Phaseolus. Protoplasma 93: 363–367CrossRefGoogle Scholar
  46. 46.
    WEBB KJ 1982 Growth and cytodifferentiation in cell suspension cultures of Pinus contorta. In Proc IUFRO Sect S2 01 5. Int Workshop In Vitro“ Cultivation For Tree Species, Fontainebleau, France, 1981, pp 217–226Google Scholar
  47. 47.
    WETMORE RH, S SOROKIN 1955 On the differentiation of xylem. J Arnold Arbor 36: 305–324Google Scholar
  48. 48.
    WETMORE RH, JP RIER 1963 Experimental induction of vascular tissues in callus of angiosperms. Am J Bot 50: 418–430CrossRefGoogle Scholar
  49. 49.
    WHITE PR 1967 Some aspects of differentiation in cells of Picea glauca cultivated in vitro. Am J Bot 54: 334–353Google Scholar
  50. 50.
    WHITE PR, SN GILBEY 1966 Sources of nitrogen for spruce tissue culture. Physiol Plant 19: 177–186CrossRefGoogle Scholar
  51. 51.
    WITHERS LA 1976 Studies on the growth in culture of plant cells. XXI. Fine structural features of Acer pseudoplatanus L. cells responding to 2,4-dichlorophenoxyacetic acid withdrawal in turbidostat culture. J Exp Bot 27: 1073–1084CrossRefGoogle Scholar
  52. 52.
    WOODING FBP 1968 Fine structure of callus phloem in Pinus pinea. Planta 83: 99–110Google Scholar

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© Springer Science+Business Media Dordrecht 1987

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  • Liisa Kaarina Simola

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