Metabolic and Non-Metabolic Roles of Carbohydrates

  • Michael R. Thompson
  • Trevor A. Thorpe
Part of the Forestry Sciences book series (FOSC, volume 24-26)


Cultured plant cells require a source of carbohydrate. The pioneering studies of Haberlandt (53) indicated that sucrose improved the viability of isolated cells. Such studies stimulated later developments in plant tissue cultures (28, 125), thus allowing research into the carbohydrate nutrition of callus (29, 30, 39) and isolated cells and cell colonies (23, 24). These studies established an absolute requirement for an exogenous supply of carbohydrate as a carbon and energy source in cultured plant tissues. However, more recent studies have shown that photoautotrophic cultures can be established (7, 128, 129). The generation of photoautotrophic cultures requires cell selection and manipulation of hormone and carbon dioxide levels (7). Consequently the development of photoautotrophism may be incompatible with many applications of plant tissue culture technology, themselves requiring specific hormone levels and balances, and thus we may regard the exogenous carbohydrate supply as essential for successful culture.


Adventitious Root Zygotic Embryo Plant Tissue Culture Physiol Plant Sieve Element 
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© Springer Science+Business Media Dordrecht 1987

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  • Michael R. Thompson
  • Trevor A. Thorpe

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