Technological Advancement in Cancer Stem Cell Research

  • Tanu Sharma
  • Chandi C. Mandal


Cancer stem cells (CSCs) display a significant role in cancer research, evidenced from past decade studies. Although, with the passage of time, effective cancer therapy has been developed, still up to now, cancer possesses the second highest mortality worldwide. The only defined characteristic for every therapy failure is the presence of cells with self-renewable capacity known as cancer stem cells in the heterogeneous population of tumor. These CSCs provide a tumor resistance against various therapies like chemotherapy and radiotherapy. Thus, to prolong survival time period of cancer patients, it is prerequisite to eliminate CSC population. Thus, to develop novel effective therapeutics against primary tumors, isolation and characterization of CSCs will provide a novel insight to develop cancer therapeutics. Thus, various in vitro and in vivo approaches have been developed to isolate and target CSCs. In this chapter, we will discuss about how researchers have developed various powerful tools to characterize CSCs to develop better therapeutics to target CSCs and thus cancer and also how technology has sprung up to generate advanced preclinical models of human tumors.


Cancer stem cells (CSCs) Spheroids Organoids 



Conflict of interest: Authors have declared no conflict of interest for this book chapter.


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Copyright information

© Springer Nature Singapore Pte Ltd. 2020

Authors and Affiliations

  • Tanu Sharma
    • 1
  • Chandi C. Mandal
    • 1
  1. 1.Department of BiochemistryCentral University of RajasthanAjmerIndia

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