Histomorphometric Analysis of Anti-Aging Properties on Rat Skin

  • Idha KusumawatiEmail author
  • Kresma Oky Kurniawan
  • Subhan Rullyansyah
  • Eka Pramyrtha Hestianah
Part of the Methods in Molecular Biology book series (MIMB, volume 2138)


We present a histomorphometric analysis to enable quantitative measurement of anti-aging activity of topical substances in rat skin. In this method, the measured parameters were closely related to changes that occur on the skin caused by exposure to UV light. We used this protocol to determine the anti-aging activities of an ethanolic extract of Curcuma heyneana rhizome and retinoic acid by a measurement of the changes in epidermal thickness, the number of sunburned cells, the number of fibroblasts, and the space between collagen fibers.

Key words

Anti-aging assay Curcuma heyneana rhizome Histomorphometric analysis Skin UV rays 



This work was supported by an International Research Collaboration and Scientific Publication grant from the Ministry of Research, Technology, and Higher Education, Republic of Indonesia (Grant no. 597/UN3.14/LT/2017). Thanks to PT. Rembaka for the drug reference used in the in vivo assay. Also, thanks to all my student for the nice pictures.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Idha Kusumawati
    • 1
    Email author
  • Kresma Oky Kurniawan
    • 1
  • Subhan Rullyansyah
    • 1
  • Eka Pramyrtha Hestianah
    • 2
  1. 1.Pharmacognosy and Phytochemistry Department, Faculty of PharmacyAirlangga UniversitySurabayaIndonesia
  2. 2.Veterinary Anatomy Department, Faculty of VeterinaryAirlangga UniversitySurabayaIndonesia

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