3D Bioprinting pp 171-181 | Cite as

3D Coaxial Bioprinting of Vasculature

  • Yang Wu
  • Yahui Zhang
  • Yin Yu
  • Ibrahim T. OzbolatEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2140)


Development of a suitable vascular network for an efficient mass exchange is crucial to generate three-dimensional (3D) viable and functional thick construct in tissue engineering. Different technologies have been reported for the fabrication of vasculature conduits, such as decellularized tissues and biomaterial-based blood vessels. Recently, bioprinting has also been considered as a promising method in vascular tissue engineering. In this work, human umbilical vein smooth muscle cells (HUVSMCs) were encapsulated in sodium alginate and printed in the form of vasculature conduits using a coaxial nozzle deposition system. Protocols for cell encapsulation and 3D bioprinting are presented. Investigations including dehydration, swelling, degradation characteristics, and patency, permeability, and mechanical properties were also performed and presented to the reader. In addition, in vitro studies such as cell viability and evaluation of extra cellular matrix deposition were performed.

Key words

Vascular Tissue engineering 3D bioprinting Human umbilical vein smooth muscle cells Sodium alginate 



This work has been supported by National Science Foundation Award #1624515, the National Institutes of Health, and the Institute for Clinical and Translational Science under Grant ULIRR024979.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Yang Wu
    • 1
    • 2
    • 3
  • Yahui Zhang
    • 4
  • Yin Yu
    • 5
    • 6
  • Ibrahim T. Ozbolat
    • 1
    • 2
    • 7
    • 8
    Email author
  1. 1.Engineering Science and Mechanics DepartmentPenn State UniversityUniversity ParkUSA
  2. 2.The Huck Institutes of the Life SciencesPenn State UniversityUniversity ParkUSA
  3. 3.School of Mechanical Engineering and AutomationHarbin Institute of Technology (Shenzhen)ShenzhenChina
  4. 4.Adhezion Biomedical, LLCWyomissingUSA
  5. 5.Institute for Synthetic Biology, Shenzhen Institutes of Advanced TechnologyChinese Academy of SciencesShenzhenPeople’s Republic of China
  6. 6.University of Chinese Academy of SciencesBeijingPeople’s Republic of China
  7. 7.Biomedical Engineering DepartmentPenn State UniversityUniversity ParkUSA
  8. 8.Materials Research InstitutePenn State UniversityUniversity ParkUSA

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