Development of a suitable vascular network for an efficient mass exchange is crucial to generate three-dimensional (3D) viable and functional thick construct in tissue engineering. Different technologies have been reported for the fabrication of vasculature conduits, such as decellularized tissues and biomaterial-based blood vessels. Recently, bioprinting has also been considered as a promising method in vascular tissue engineering. In this work, human umbilical vein smooth muscle cells (HUVSMCs) were encapsulated in sodium alginate and printed in the form of vasculature conduits using a coaxial nozzle deposition system. Protocols for cell encapsulation and 3D bioprinting are presented. Investigations including dehydration, swelling, degradation characteristics, and patency, permeability, and mechanical properties were also performed and presented to the reader. In addition, in vitro studies such as cell viability and evaluation of extra cellular matrix deposition were performed.
Vascular Tissue engineering 3D bioprinting Human umbilical vein smooth muscle cells Sodium alginate
This is a preview of subscription content, log in to check access.
Springer Nature is developing a new tool to find and evaluate Protocols. Learn more
This work has been supported by National Science Foundation Award #1624515, the National Institutes of Health, and the Institute for Clinical and Translational Science under Grant ULIRR024979.
Frueh FS, Menger MD, Lindenblatt N, Giovanoli P, Laschke MW (2017) Current and emerging vascularization strategies in skin tissue engineering. Crit Rev Biotechnol 37:613–625CrossRefGoogle Scholar
Chen M, Przyborowski M, Berthiaume F (2009) Stem cells for skin tissue engineering and wound healing. Crit Rev Biomed Eng 37:399–421CrossRefGoogle Scholar
Wu Y, Wong YS, Fuh JYH (2017) Degradation behaviors of geometric cues and mechanical properties in a 3D scaffold for tendon repair. J Biomed Mater Res A 105:1138–1149CrossRefGoogle Scholar
Dahl SLM, Koh J, Prabhakar V, Niklason LE (2003) Decellularized native and engineered arterial scaffolds for transplantation. Cell Transplant 12:659–666CrossRefGoogle Scholar
Canham PB, Talman EA, Finlay HM, Dixon JG (1991) Medial collagen organization in human arteries of the heart and brain by polarized light microscopy. Connect Tissue Res 26:121–134CrossRefGoogle Scholar
Caves JM, Kumar VA, Martinez AW, Kim J, Ripberger CM, Haller CA et al (2010) The use of microfiber composites of elastin-like protein matrix reinforced with synthetic collagen in the design of vascular grafts. Biomaterials 31:7175–7182CrossRefGoogle Scholar
Salerno A, Zeppetelli S, Di Maio E, Iannace S, Netti PA (2011) Processing/structure/property relationship of multi-scaled PCL and PCL-HA composite scaffolds prepared via gas foaming and NaCl reverse templating. Biotechnol Bioeng 108:963–976CrossRefGoogle Scholar
Norotte C, Marga F, Niklason L, Forgacs G (2010) Scaffold-free vascular tissue engineering using bioprinting. Biomaterials 30:5910–5917CrossRefGoogle Scholar
Zhang Y, Yu Y, Akkouch A, Dababneh A, Dolati F, Ozbolat I (2015) In vitro study of directly bioprinted perfusable vasculature conduits. Biomater Sci 3:134–143CrossRefGoogle Scholar
Yu Y, Moncai K, Li J, Peng W, Rivero I, Martin JA, Ozbolat I (2016) Three-dimensional bioprinting using self-assembling scalable scaffold-free ‘tissue strands’ as a new bioink. Sci Rep 6:28714CrossRefGoogle Scholar
Akkouch A, Yu Y, Ozbolat IT (2015) Microfabrication of scaffold-free tissue strands for three-dimensional tissue engineering. Biofabrication 7(3):031002CrossRefGoogle Scholar