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Super-Resolution Microscopy and Particle-Tracking Approaches for the Study of Vesicular Trafficking in Primary Neutrophils

  • Jennifer L. Johnson
  • Kersi Pestonjamasp
  • William B. Kiosses
  • Sergio D. CatzEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2233)

Abstract

Neutrophils are short-lived cells after isolation. The analysis of neutrophil vesicular trafficking requires rapid and gentle handling. Recently developed super-resolution microscopy technologies have generated unparalleled opportunities to help understand the molecular mechanisms regulating neutrophil vesicular trafficking, exocytosis, and associated functions at the molecular level. Here, we describe super-resolution and total internal reflection fluorescence (TIRF) microscopy approaches for the analysis of vesicular trafficking and associated functions of primary neutrophils.

Key words

Total internal reflection fluorescence (TIRF) Vesicular trafficking Neutrophils 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2021

Authors and Affiliations

  • Jennifer L. Johnson
    • 1
  • Kersi Pestonjamasp
    • 1
  • William B. Kiosses
    • 2
  • Sergio D. Catz
    • 1
    Email author
  1. 1.Department of Molecular MedicineThe Scripps Research InstituteLa JollaUSA
  2. 2.Department of Vascular and Cell BiologyLa Jolla Institute for ImmunologyLa JollaUSA

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