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Measurements of Compensatory Endocytosis by Antibody Internalization and Quantification of Endocytic Vesicle Distribution in Adrenal Chromaffin Cells

  • Mara Ceridono
  • Sylvette Chasserot-Golaz
  • Nicolas Vitale
  • Stéphane Gasman
  • Stéphane OryEmail author
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Part of the Methods in Molecular Biology book series (MIMB, volume 2233)

Abstract

Plasma membrane proteins are amenable to endocytosis assays since they are easily labeled by reagents applied in the extracellular medium. This has been widely exploited to study constitutive endocytosis or ligand-induced receptor endocytosis. Compensatory endocytosis is the mechanism by which components of secretory vesicles are retrieved after vesicle fusion with the plasma membrane in response to cell stimulation and a rise in intracellular calcium. Luminal membrane proteins from secretory vesicles are therefore transiently exposed at the plasma membrane. Here, we described an antibody-based method to monitor compensatory endocytosis in chromaffin cells and present an image-based analysis to quantify endocytic vesicles distribution.

Key words

Compensatory endocytosis Large dense core vesicle Neuroendocrine cells Chromaffin cells Internalization Dopamine-β-hydroxylase 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2021

Authors and Affiliations

  • Mara Ceridono
    • 1
  • Sylvette Chasserot-Golaz
    • 1
  • Nicolas Vitale
    • 1
  • Stéphane Gasman
    • 1
  • Stéphane Ory
    • 1
    Email author
  1. 1.Centre National de la Recherche ScientifiqueUniversité de Strasbourg, Institut des Neurosciences Cellulaires et IntégrativesStrasbourgFrance

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