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Methods for Monitoring Endocytosis in Astrocytes

  • Maja Potokar
  • Jernej Jorgačevski
  • Robert ZorecEmail author
Protocol
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Part of the Methods in Molecular Biology book series (MIMB, volume 2233)

Abstract

Endocytosis is a vesicle-based mechanism by which eukaryotic cells internalize extracellular material. There are several types of this universal mechanism linked to different types of endocytosed cargo, including pathogens; therefore, several approaches can be applied. Here, we describe techniques that are applicable to study the internalization of flaviviruses; dextrans; transporters, such as, glutamate transporter vGlut1; and peptidergic signaling molecules, including atrial natriuretic peptide into astrocytes, the most heterogeneous neuroglial cells, which play a key homeostatic role in the central nervous system.

Key words

Endocytosis Recycling vesicles Astrocyte Flavivirus vGlut1 ANP Gliotransmitter Dextran 

Notes

Acknowledgments

This work was supported by the Research Agency of Slovenia (grant numbers P3 0310, J3 9266, and J3 7605), BI-JP/18-20-002, and EuroCellNet COST Action (CA15214).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2021

Authors and Affiliations

  • Maja Potokar
    • 1
    • 2
  • Jernej Jorgačevski
    • 1
    • 2
  • Robert Zorec
    • 1
    • 2
    Email author
  1. 1.Laboratory of Neuroendocrinology—Molecular Cell Physiology, Institute of Pathophysiology, Faculty of MedicineUniversity of LjubljanaLjubljanaSlovenia
  2. 2.Celica BIOMEDICALLjubljanaSlovenia

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